Assembly worked at last

Today in lab I did a miniprep of yesterday's cell pellets using a completely different purification kit. The new procedure was straightforward and went well overall. (The handbook even gave me a new tip on easily resuspending cells.) I was relieved to find that the results had great DNA concentrations relative to the volume I eluted in. The cultures grown in small tubes had lower yield as expected, with one set on the edge of useful concentration, but the purification clearly worked. Sometime next week we'll send these plasmids for sequencing.