Running numbers for the lab

I'm continuing to help perform experiments in the lab, but recently I've also had the opportunity to do some data analysis on a set of aligned mRNA sequences. We're trying to determine what proportion of this protein's instances are from a certain splice variant. One might think this would be easy to detect in aligned sequences (especially since there's a consensus sequence that they're aligned to), but due to imperfect sequencing technology, there are some judgment calls. Nevertheless, I have some possible numbers that seem right from eyeballing a visual representation of the alignments. I will continue to work on making refinements and looking for interesting related metrics.