Thursday, January 18, 2018

Lab work begins

Today I started doing some actual lab work with my supervisor. We set up a PCR with multiple primers to make copies of just a few specific runs of DNA. We will next use Gibson assembly to produce unified, rearranged plasmids from those runs. My job was to pipette a lot of very small amounts of liquids into various tubes to prepare the 18 (2 templates times 3 temperatures times 3 primers) PCR tubes. There's a lot to keep track of, and though some parts were repetitive, it was pretty neat getting to actually set up a PCR, having only read about it previously. I also got to start a lab notebook and start keeping track of relevant information.

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