Back to lab

This afternoon I went into lab. Sequencing results came in a while back for my Gibson assembly products, so I examined those. A couple seem to have worked perfectly fine, but not all. On one plasmid, the region of reliable sequence didn't quite reach the region of interest, so I don't know for sure whether my desired deletion was achieved. On another, the deletion was perfect but a random single-base change happened to produce a stop codon, which would terminate the protein early. Even the good plasmids had one or two single-base changes, but nothing catastrophic.